Even at low ISO, if you are taking a long exposure, especially where it is warmer, it is an issue, because of thermal heat/signal. Astrophotographers have to deal with this all the time (at least those that use a non-dedicated astro CCD camera). Some have made homemade peltier cooling devices to help thwart this problem.
Yes, you can use the function in your camera, as others have already said though, it takes twice the amount of time, since your camera is taking the original exposure, and then closing off the shutter to take the dark frame exposure.
What some astroimagers do is, as long as ambient temp is roughly the same the whole time, is take their dark frames (calibration frames), at the end of imaging for the night. And then either use editing program or special astro program (like, Images Plus), to do the dark frame subtraction. Just make sure temp is the same, length of time is the same, and same ISO is used. As well as taking more shorter exposures and stacking them, which is a good way to do it, whether taking astroimages or not. You get more signal that way... the good kind.
Either way - dark subtraction does help, unless of course you are using a Starlight Express, SXV-M25. And then, you don't need dark subtraction!